Drug identification
Character identification
(1) The five columns of root bark are irregular and Zheng or Zheng Zheng barrel-like, some are piece-shaped, length 4-15cm, diameter 0.5-1.5cm, thickness 1-4mm. The outer surface is gray-brown or gray-brown, with irregular cracks or longitudinal wrinkles and long lenticels; the inner surface is white or grayish yellow with fine vertical stripes. Lightweight, crisp, irregular section, gray or grayish yellow. The gas is slightly fragrant and the taste is slightly spicy and bitter.
It is better to have thick skin, aroma, and grayish white cross-section.
(2) Acanthus quinquefolium is cylindrical or cut into irregular pieces. Thick skin 0.5-1mm. The outer surface is gray-brown and brown, with longitudinal wrinkles, light lenticels, and lateral bulges. Bark dark gray or gray-black; tender stems with thorns, a flat cone, most spalled, punctate or radial oval punctate. Gas is slightly fragrant and tasteless.
Microscopic identification
(1) Transverse section of fine root and root bark: 7-14 rows of cork. Cortical cells 2-4 columns scattered with resin. The phloem ray is 1-8 cells wide, the phloem resin channels are arranged in a 3-5(-8) ring layer, the resin channels are oval or oval, the major diameter is 60-225(-420) μm, and the diameter is 14- 24 μm, 4-5 peripheral secretory cells, oblong and rectangular, containing light yellowish secretions, the medial resinous tracts are mostly round and 14-20 μm in diameter. Cortical and phloem parenchyma cells containing calcium oxalate clusters have a diameter of 12-60 μm, coarse corners, few sharp tips, small clusters of ray cells, and constant or dozens of radial alignments. Parenchyma cells contain starch grains, single oval or spheroidal, diameter 8-14μm. The bast fibers in the old root bark have a length of 240-600 μm, and the cells are narrow and the hole Han is obvious.
(2) sessile five plus root bark cross section: cork layer 12-20. Cortical 3-4 columns. The phloem ray has a width of 1-3 rows of cells, a wavy curve, a cell wall with a reticular texture, a plurality of phloem resin pathways, an elliptical or circular shape, a major diameter of 40-116 μm, and 4-8 peripheral secretory cells of a diameter of 32-50 μm. In parenchyma cells, clusters of 8-20 (-40) μm phloem occasionally have single or 2-7 bundled fibers scattered. The fiber length is 220-600μm, diameter is 20-50μm, and the pores are obvious.
Bark cross-section: cork layer thickness of 20-25 columns, some cork cell wall thickening was stratified. Thick-corner cells 5-6 columns. There are fiber bundles in the phloem, scattered between the rays, fiber length 600-1500 (-2000) μm, fewer resin tracts, and more distributed in the outside of fiber bundles, parenchyma cell clusters less than root bark.
YESU Y41SE Fluorescent Detection Kit
1. The six-color fluorescence detection platform amplified 40 Y chromosome STR loci simultaneously, including 33 low mutation rate STR loci (mutation rate less than 1%) and 7 fast mutation loci (mutation rate more than 1%), a very low mutation rate Y INDEl
2. Y-str database is suitable for male pedigree screening and has strong individual identification ability. It contains all 35 loci which are required and preferred in Y database construction criteria.
3. Contains 12 mini-STR(loci with amplified length less than 220bp) to obtain more information from DNA degradation materials.
4, material compatibility, compatible with the direct amplification of most materials and the amplification of purified DNA
5. Rapid amplification, the theoretical amplification time was about 70 minutes.
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