1. The culturing room should be kept clean, well ventilated, and dimly lit; the room temperature should be kept at about 22°C, and the relative humidity of the air should be 65-70%.
2, the use of high temperatures at noon every day to open doors and windows for ventilation for half an hour, usually closed doors and windows should pay attention to insulation.
3. When the inoculation mouth mycelium expands about 5cm, the first turning is performed. At this time, the bacteria sticks are adjusted up and down in order to absorb oxygen evenly and make bacteria consistent.
4. When the mycelium is close to the connecting port, the second turn is performed. This time, the bacterial rods are drilled to increase the oxygen. The method is to use a 5cm long nail to make a triangular punch plate to inoculate the bacteria. Sting around the mouth, about 18 holes per stick. The bacteria bar can improve the oxygen content in the matrix of the culture medium by slicing holes, enhance the metabolic function and growth activity of the mycelium, and promote the decomposition and transformation of nutrients by the mycelia and accumulation of nutrients. The mycelium metabolism starts to accelerate at this time, and the bag temperature is usually 3-5°C higher than room temperature. If the room temperature continues to be higher than 26°C, it should be divided into compartments.
5. When the mycelium grows full, carry out the third turn and the second time. This time, 12 to 15 cm long nails were used to make a perforated plate equal to the length of the fungus rod in two rows. The holes were punched at the same time on both sides of the fungus bar. The fungus bar was pierced and turned over. In 70 days, when most of the brown pellicle membranes appeared on the surface of the mushroom bag, it indicated that the bag entered the reproductive growth stage, that is, the field was transformed into a mushroom.
Intelligence Electric Product,Best Chatbot ,Google Chatbot ,Tara Chatbot
ChangChun E-vida Technology Co.,ltd , https://www.evidatech.com