High performance liquid chromatography (HPLC) is a method for qualitative and quantitative analysis of substances. It has the characteristics of fast analysis speed, high separation efficiency and automation of operation. High performance liquid chromatography is widely used. A range of instruments that can be used in high performance liquid chromatography pretreatment.
1. The reagents used in the mobile phase of the mobile phase generally use analytically pure reagents. The water is preferably ultrapure water or double distilled water; the mobile phase is used to remove particles that may be present before use. With a solvent filter, it is essential to have a vacuum source - a vacuum pump. When there are bubbles in the mobile phase, it will cause noise peaks on the chromatogram. In severe cases, the sensitivity of the analysis will decrease. When the bubbles become larger and enter the flow path or the column, the flow velocity will be slow or unstable, and the baseline will be made. ups and downs. The use of an in-line degasser continuously removes air bubbles from the mobile phase.
2. Inject the sample The sample is applied with ultrasonic waves to make it evenly mixed. After filtering through the filter, it enters the column.
3. When the check valve is in use, there is often a phenomenon that the inlet and outlet check valves fail. The reason may be that the one-way valve is gambling and can be solved by ultrasonic cleaning.
4. The column oven can effectively keep the column temperature constant, preventing drift in retention time.
We can see that pre-laboratory instruments can be widely used in pre-experimental processing of high performance liquid chromatography.
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